The complete nucleotide sequence of the secretable alpha-amylase gene ALP1 from the yeast Saccharomycopsis fibuligera has been determined. The ALP1 DNA hybridized to a polyadenylated RNA of 2.0 kilobases. A single open reading frame encodes a 494-amino acid protein which is highly homologous with alpha-amylase (Taka-amylase) of a fungus Aspergillus oryzae.