The 3' terminal region from the LEU2 gene of yeast has been sequenced. Two open reading frames (ORF) have been identified, one of which constitutes the 3' terminus of beta-isopropylmalate dehydrogenase, the product of the LEU2 gene. A noncoding spacer region located between the ORFs contains two consensus-type transcriptional terminators. The terminator-like sequences are oriented in opposing directions on opposite DNA strands. The noncoding spacer region may represent a single terminator for the LEU2 gene or two separate terminators involved in blocking convergent transcription from an as yet unidentified yeast gene.