cDNA encoding the beta isoform of human phosphatidylinositol transfer protein was cloned from a human brain cDNA library. The deduced sequence of the protein comprised 271 amino acids with a calculated molecular mass of 31,539 Da, and showed 98.1% identity to that of the beta isoform of rat phosphatidylinositol transfer protein. The cDNA hybridized to a 3.4-kb mRNA, which was widely expressed in various human tissues including brain.