design.matrix[,1]+design.matrix[,2]+design.matrix[,3]+design.matrix[,4]+design.matrix[,5]+design.matrix[,6]+design.matrix[,7]+design.matrix[,8]+design.matrix[,9]+design.matrix[,10]+design.matrix[,11]+design.matrix[,12]+design.matrix[,13]+design.matrix[,14]+design.matrix[,15]+design.matrix[,16]+design.matrix[,17]+design.matrix[,18]+design.matrix[,19]+design.matrix[,20]+design.matrix[,21]+design.matrix[,22]+design.matrix[,23]+design.matrix[,24]+design.matrix[,25]+design.matrix[,26]+design.matrix[,27]+design.matrix[,28]+design.matrix[,29]+design.matrix[,30]+design.matrix[,31]+design.matrix[,32]+design.matrix[,33]+design.matrix[,34]+design.matrix[,35]+design.matrix[,36]+design.matrix[,37]+design.matrix[,38]+design.matrix[,39]+design.matrix[,40]+design.matrix[,41]+design.matrix[,42]+design.matrix[,43]+design.matrix[,44]+design.matrix[,45]+design.matrix[,46]+design.matrix[,47]+design.matrix[,48]+design.matrix[,49]+design.matrix[,50]+design.matrix[,51]+design.matrix[,52]+design.matrix[,53]+design.matrix[,54]+design.matrix[,55]+design.matrix[,56]+design.matrix[,57]+design.matrix[,58]+design.matrix[,59]+design.matrix[,60]+design.matrix[,61]+design.matrix[,62]+design.matrix[,63]+design.matrix[,64]+design.matrix[,65]+design.matrix[,66]+design.matrix[,67]+design.matrix[,68]+design.matrix[,69]+design.matrix[,70]+design.matrix[,71]+design.matrix[,72]+design.matrix[,73]+design.matrix[,74]+design.matrix[,75]+design.matrix[,76]+design.matrix[,77]+design.matrix[,78]+design.matrix[,79]+design.matrix[,80]+design.matrix[,81]+design.matrix[,82]+design.matrix[,83]+design.matrix[,84]+design.matrix[,85]+design.matrix[,86]+design.matrix[,87]+design.matrix[,88]+design.matrix[,89]+design.matrix[,90]+design.matrix[,91]+design.matrix[,92]+design.matrix[,93]+design.matrix[,94]+design.matrix[,95]+design.matrix[,96]+design.matrix[,97]+design.matrix[,98]+design.matrix[,99]+design.matrix[,100]+design.matrix[,101]+design.matrix[,102]+design.matrix[,103]+design.matrix[,104]+design.matrix[,105]+design.matrix[,106]+design.matrix[,107]+design.matrix[,108]+design.matrix[,109]+design.matrix[,110]+design.matrix[,111]+design.matrix[,112]+design.matrix[,113]+design.matrix[,114]+design.matrix[,115]+design.matrix[,116]+design.matrix[,117]+design.matrix[,118]+design.matrix[,119]+design.matrix[,120]+design.matrix[,121]+design.matrix[,122]+design.matrix[,123]+design.matrix[,124]+design.matrix[,125]+design.matrix[,126]+design.matrix[,127]+design.matrix[,128]+design.matrix[,129]+design.matrix[,130]+design.matrix[,131]+design.matrix[,132]+design.matrix[,133]+design.matrix[,134]+design.matrix[,135]+design.matrix[,136]+design.matrix[,137]+design.matrix[,138]+design.matrix[,139]+design.matrix[,140]+design.matrix[,141]+design.matrix[,142]+design.matrix[,143]+design.matrix[,144]+design.matrix[,145]+design.matrix[,146]+design.matrix[,147]+design.matrix[,148]+design.matrix[,149]+design.matrix[,150]+design.matrix[,151]+design.matrix[,152]+design.matrix[,153]+design.matrix[,154]+design.matrix[,155]+design.matrix[,156]+design.matrix[,157]+design.matrix[,158]+design.matrix[,159]+design.matrix[,160]+design.matrix[,161]+design.matrix[,162]+design.matrix[,163]+design.matrix[,164]+design.matrix[,165]+design.matrix[,166]+design.matrix[,167]+design.matrix[,168]+design.matrix[,169]+design.matrix[,170]+design.matrix[,171]+design.matrix[,172]+design.matrix[,173]+design.matrix[,174]+design.matrix[,175]+design.matrix[,176]